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Pesticide Not Required centres around a farm where the player must fend off hordes of incoming insects which increase in difficulty during the duration of the level. The player can choose from one of many frogs, all of which have different stats. Pesticide Not Required differs from other roguelites and bullet heaven games with its emphasis on light farm-sim elements. Located in the centre of the map is a plot of land which is used to sow plant seeds and weapon seeds. Both seed types require water and time to grow. Water can be collected from two water wells near the farming plot. Seeds give the player XP, weapons allow the player to upgrade equipped weapons. Similar to other roguelite games, weapon upgrades and level upgrades (earnt from XP) give players a choice between different stats and upgrades. Level upgrades offer the player a choice between 3 perks, some with negative stats and some with positive stats. The three options can be re-rolled once if the player is not satisfied with the first selection. The levels run on a day and night cycle, with a shop opening up at the end of each day / horde wave. Seeds, weapons, plot expansions, and even pets can be purchased through the shop. Currency is earnt and collected by killing enemy insects or from mining the various rocks around the map. Pets can do a variety of things such as water plants, fertilise plants, plant seeds, and attack enemies. Once a level is complete the player can return to their home where they can select different frog characters and change the games level/stage. Levels or stages are represented as seasons.